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Description
Human STAT4 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Signal Transducer and Activator of Transcription 4 (STAT4) capture antibody. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Signal Transducer and Activator of Transcription 4 (STAT4) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Signal Transducer And Activator Of Transcription 4 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Signal transducer and activator of transcription 4 (STAT4) is a transcription factor belonging to the STAT protein family, which consists of STAT1, STAT2, STAT3, STAT5A, STAT5B, and STAT6. STAT proteins are key activators of gene transcription, binding to DNA in response to cytokine gradients. STAT proteins are common components of the Janus kinase (JAK) signaling pathway and are activated by cytokines. STAT4 is required for the development of naive CD4+ T cells into Th1 cells and for the production of interferon-γ in response to IL-12. There are two known STAT4 transcripts, STAT4α and STAT4β, which differ in their downstream interferon-γ (IFN-γ) production. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
Based on 973 reviews
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Product Reviews
★★★★★ 5
Cheaper at homegoods
Color: Gray/Blue, Size: Small
It works great. I saw them at homegoods for a dollar cheaper.
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Reviewed in the United States on February 21, 2026
★★★★★ 3
Not the best
Color: Beige, Size: Large
Not the best fabric shaver I’ve used, by a long shot. While I appreciate the shield for protecting expensive fabrics, it stands in the way of being able to effectively use this. I have some thinner cashmere and merino sweaters with some pilling that I was really hoping to fix and this didn’t do a good job and was more of a headache due to the shield problem. The lint roller is okay but not as much adhesive as others I have so is not as efficient at picking up lint and requires multiple rounds to remove the lint. It’s lightweight and straightforward to use but wish it worked better. This is okay for the price but spending a few bucks more will get you a set that’s more effective, which is worth it in the long run to save you time on what should really otherwise be simples tasks to do.
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Reviewed in the United States on April 7, 2026
★★★★★ 5
Works great and lovely color!
Color: Beige, Size: Large, Color: Beige, Size: Large
A decent lint remover and great fabric shaver. I have so many tops that bead and this fabric shaver has become a most effective way to remove those little fuzzies and beaded areas of fabric that make my tops look old. This makes my clothes look new. It’s extremely easy to use but it does not include the 2 AA batteries you’ll need, so definitely grab you some. It’s durable and does the job fast. Overall, very handy, a good value, and excellent quality. I would recommend!
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Reviewed in the United States on February 27, 2026
★★★★★ 2
pretty junk-y. worst lint roller ever created.
Color: Sage, Size: Large, Color: Sage, Size: Large
My review is for the
Farberware Fabric Shaver & Lint Remover Set–Battery Operated Sweater Shaver with Triple Blades and Extra Sticky Roller for Pet Hair, Fuzz & Pilling on Clothes, Upholstery & Bedding (Sage, Large) (Sage, Large)
Honestly, this is one of the worst fabric shavers I've used. It is also absolutely the worst lint roller I have ever used. And I've used the IKEA ones that are even much cheaper than this.
Absolutely do not push us this, unless you make your life more difficult or find that you have no benefit to your clothes whatsoever.
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Reviewed in the United States on February 13, 2026
★★★★★ 4
It shouldn’t be this hard to use, don’t like the fabric guard
Color: Gray/Blue, Size: Large
This review is for the Best Brands listing “Farberware Large Lint Remover & Fabric Shaver Set – Battery Powered Sweater Shaver with Triple Blades and Extra Sticky Roller for Pet Hair, Fuzz & Pilling on Clothes & Furniture“.
This set contains a fabric shaver as well as a lint removal roller. The fabric shaver needs batteries, not included, and the orientation is weirdly not marked on the side that takes the batteries but instead on the outside of the battery lid, on the last surface you’d check. So even though this needed a very normal two AA batteries it was still harder to install than it needed to be. Also, emptying the lint collector is harder than it needs to be. I need to use both hands and hold the remover against my body to detach the lint container. It comes off with a “click” that does not bode well for the durability of the device.
As for using the lint remover, it does an ok job. It has a fabric guard to presumably prevent anyone creating holes in the fabric they’re cleaning and the guard can be lowered/raised slightly. However, it keeps the lint remover far enough away from the fabric you need to press pretty hard to get decent results and you can’t get as close as you need in some areas due to the bulk of the plastic guard. The handle is also a little bulky, it’s like they made the entire device larger instead of just making the shaving surface and the lint catcher larger.
The lint roller was pretty standard. I have long nails and usually struggle with detailed tasks, but I was able to get to the sticky panels without too much hassle. Although u made a bit of a mess trying to get the lint roller started, the roller was nicely sticky and lasted a little longer than some cheaper alternatives. I was able to get the sticky sheet to tear at the perforated edge easily.
I have used the shaver to clean an old wool sweater as well as a newer pair of jersey sweatpants, and the results were ok but I prefer my smaller, older lint remover for effectiveness and ease of use. The lint roller was very nice, although I sort of expected the pretty pattern to be on the entire roll not just the front cover. This set works ok, but I really don’t like the lint remover and am removing a star because it’s harder to use than it should be, including the presence of the bulky fabric guard.
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Reviewed in the United States on December 25, 2025